Figure 6.
Number of Leydig cells (HSD3B immunopositive)/testis (top panel), and corresponding changes in their nuclear diameter (bottom panel) in juvenile monkeys treated for 11 days with FSH, LH or FSH+LH. HSD3B positive cells were absent in the vehicle (VEH) treated group. Treatment with LH, either alone or in combination with FSH, resulted in a significant increase in the number of Leydig cells (top panel). Interestingly, FSH was able to also stimulate the expression of this enzyme although the effect was not statistically significant by ANOVA (top panel). A significant increase in nuclear diameter of Leydig cells was noted with LH stimulation, either alone or in combination with FSH (bottom panel). Note the nuclear diameter was not determined in the VEH group (bottom panel) since HSD3B immunoactivity was absent. a, P ≤0.05 from the remaining groups and b, from FSH group.