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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Glia. 2014 Jul 17;62(12):2034–2043. doi: 10.1002/glia.22724

Figure 5.

Figure 5

Post-treatment with subpicomolar DPI attenuates NOX2 activation induced by LPS through detachment of p47phox from the plasma membrane. (A) Midbrain neuron-glia cultures were pre-treated with LPS for 12 h, followed by DPI (10−14 or 10−13 M) treatment. Superoxide production was significantly inhibited by subpicomolar DPI post-treatment. (B) Western blot analysis revealed that subpicomolar DPI post-treatment detaches p47phox from the plasma membrane in LPS-treated HAPI microglia cells (gp91phox as an internal membrane control). The densities of the membrane p47phox signals were quantified. The results are expressed as a percentage of the LPS group (mean ± SEM) from three to four experiments performed in duplicate and were analyzed using one-way ANOVA, followed by Bonferroni’s post hoc multiple comparison test. **p < 0.01.