Figure 8. Neurons generated following QA-induced lesions of adult zebrafish telencephalon adopt multiple phenotypes.

(A, A′) In Tg(her4:creER^T2;β-actin2:LCLG) (her4:GFP) fish at 28 days post-lesioning, cells birthdated with EdU (A; red in A′) at two days after QA and tamoxifen injection have settled in the periventricular region and parenchyma of the damaged hemisphere where many co-express GFP (green in A′). (B–B′) Higher magnification view of a GFP/HuC/D double-labeled cell with neuronal morphology in the parenchyma. (C) Neurons labeled for the inhibitory neuronal marker GABA include cells that co-express GFP that comingle closely with other GABA-expressing cells. (D–E) Neurons double-labeled (yellow arrows) for GFP (green) and tyrosine hydroxylase (TH) comingle among the distinctive TH positive cluster of cells (red) in the ipsilesional hemisphere. (F) Within a calretinin-expressing group of neurons, some cells co-expressed calretinin (red) and GFP (green) whereas neighboring cells showed immunoreactivity for one of the two markers. Scale bar A, B, D = 100 μm. Scale bar C, E, F = 50 μm.