Fig. 6.
Electron micrographs showing immunoperoxidase and immunogold–silver labeling for HA-α2A-AR in axon terminals in the mPFC. (A and D–H) Peroxidase labeling for HA-α2A-AR in axon terminals (HA-α2Art) that are in direct contact with unlabeled dendrites (ud) in the mPFC. In these synaptic contact arrangements, some HA-α2Art form visible asymmetric (curved arrow, D and E) or symmetric (zigzag arrow, F–H) synapses, whereas other appositions are difficult to unequivocally establish in the plane of section analyzed and are classified as ‘undefined’ (A and B). Some HA-α2Art are directly apposed to unlabeled axon terminals (ut) (B, F and G) or converge on common dendrites with unlabeled axon terminals (B and D). Peroxidase-labeled HA-α2A-AR axon terminals are also apposed by astrocytic processes (*) (C and D). (I) Electron micrograph showing immunogold–silver labeling (arrowheads) for HA-α2A-AR in an axon terminal that is in direct contact with an unlabeled dendrite (ud). The unlabeled dendrite receives convergent input from a ut. Scale bars, 0.5 µm.