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. Author manuscript; available in PMC: 2015 Sep 26.
Published in final edited form as: Oncogene. 2014 Apr 21;34(13):1709–1717. doi: 10.1038/onc.2014.97

Figure 3. UBQLN1 loss induces cell migration and invasion.

Figure 3

(a) Migration assay in A549 and H358 cells. Cells were transfected with either non targeting (siNT) siRNA or two different siRNAs targeting UBQLN1 (siU1 and siU1-2). 24 hrs post siRNA transfections a pipette tip was used to scratch the dish to make a “wound”. Cells were examined after wound has been formed and successively for 24hr and 48hr post wound formation and photographed. (b) Invasion assay in A549 cells. Cells were transfected with either non targeting (siNT) siRNA or two different siRNAs targeting UBQLN1 (siU1 and siU1-2). 24 hours after siRNA transfection cells were seeded into Boyden chambers without (left) or with (right) matrigel. The lower chamber contained media with serum, whereas the upper chamber containing the cells was without serum. 48 hrs later cells on the underside of the membrane were fixed and stained. (c) Quantification of relative number of cells migrated or invaded through matrigel (*P < 0.05).