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. 2014 Oct 14;14:278. doi: 10.1186/s12870-014-0278-3

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© Xu et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phenotype of silique elongation and expressional analyses of transgenes in the P _Gh80 :Gh80-32R2 and P _Gh80 :Gh80MD-32R2 Arabidopsis. A. A schematic representation of the P _Gh80 :Gh80-32R2 and P _Gh80 :Gh80MD-32R2 chimeric constructs. B and C. Wild-type Arabidopsis possessing either the P _Gh80 :Gh80-32R2 (B) or P _Gh80 :Gh80MD-32R2 (C) transgene has a partially sterile phenotype. D and E. Alexander’s staining shows the majority of pollen grains lack cytoplasm and are aborted in the P _Gh80 :Gh80-32R2 (D) and P _Gh80 :Gh80MD-32R2 (E) anthers. Scale bar = 85 μm. F and G. The relative expression levels of the P _Gh80 :Gh80-32R2 (F) and P _Gh80 :Gh80MD-32R2 (G) transgenes in the selected lines. The higher the ratio (P _Gh80 :Gh80-32R2 or P _Gh80 :Gh80MD-32R2 vs. AtMYB80), the lower the plant fertility obtained. Gh32R2, P _Gh80 :Gh80-32R2; GhMD32R2, P _Gh80 :Gh80MD-32R2; Endo80, endogenous AtMYB80. Error bar represents SD.