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Characterization of anti-Ep-CAM MAbs.
| Clone name | Isotype1 | Titer2 | FCM staining | Immunohis-tochemistry3 |
| FMU-Ep1 | IgG 2a | 1×10-6 | - | ++ |
| FMU-Ep2 | IgG 1 | 1×10-7 | - | ++ |
| FMU-Ep3 | IgG 2a | 1×10-5 | - | ++ |
| FMU-Ep4 | IgG 1 | 1×10-5 | + | + |
The isotypes of MAbs were identified by an isotyping kit (Pierce).
The titers of MAbs in ascites were measured by indirect ELISA.
Double plus (++) stands for strong positive staining and single plus (+) stands for weak positive staining.
