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Expression of smad4 protein. A: Weak intracellular expression of Smad4 protein in SW620 cells. The positive staining for Smad4 was localized in cytoplasm; B: Weak intracellular expression of Smad4 protein in PcDNA3.1-SW620 cells. The positive staining for Smad4 was localized in cytoplasm; C: Strong intracellular expression of Smad4 protein in DPC4+-SW620 cells (clone2). The positive staining for Smad4 was localized in cytoplasm and nucleus, mainly in cytoplasm.
