Figure 4.

Secretion of chemotactic factors by Hep3B cells for lymphocytes from healthy subjects in vitro. The ability of culture supernatants from Hep3B cells to induce CD⁴⁺ (A) and CD⁸⁺ (B) T cell chemotaxis was tested by chemotactic experiments as described in Materials and Methods. To test the effect of blocking CXCR3 on the chemotactic response of lymphocytes to Hep3B culture supernatants, lymphocytes were pretreated with anti-CXCR3 monoclonal antibody or IgG control before the chemotaxis assay. Results are expressed as migration index, calculated as described in Materials and Methods.