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NO-dependent DNA damage evaluated by comet assay. A: Fluorescence microscopy showing the apparent comet tail in gallbladder cells stimulated by the cytokine mixture and intact spherical nuclei of the cells in the control group and CM + L-NMMA group; B: Analysis of the proportion of DNA migrated to comet tails using NIH image showing a significantly higher level of DNA damage in the CM group than in the control group, which decreased to the control level after the addition of L-NMMA.
