Figure 5. Intact TRN microcircuit dissection connects form to function.
(A) Visual tagged neurons are positively correlated to cortical spindle power in SWS (P<10−8, signed-rank test), but (B) anterior tagged neurons are negatively correlated (P=0.006). (C) Anterior tagged neurons are wake active, while visual tagged neurons are state-indifferent. (D) Visual tagged neurons show stronger phase-locking to spindle oscillations (P<0.001, rank-sum test at the trough). (E) Visual-, but not anterior-, tagged neurons exhibit enhanced pair-wise spike-time synchrony in SWS (P-values: signed-rank test, numbers of axes denote Z-scores). (F) Visual detection task design ensures control over psychophysical parameters. The mouse is informed of a new trial by a white noise stimulus emitted from two side speakers. To initiate a trial, the mouse is required to hold its snout in a nose-poke for a period of 0.5-0.7s, ensuring that when the 0.5s stimulus is presented at one of the reward nose-pokes, the head is in the correct orientation to see it. The rotating disk ensures that the reward sites are only available following the stimulus, minimizing impulsive poking behavior. (G) Only visual tagged neurons show a reduction in firing rate (group mean ± SEM; P< 0.001, rank-sum test) during the attentional window of the visual detection task (yellow bar: stimulus). See also table S2.