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Giemsa stained metaphase (a), corresponding karyotype of Carassius carassius (b), and metaphase spread sequentially stained with AgNO3 (c) and CMA3 (d). NOR chromosomes shown in frames (in a and b), Ag-NORs and corresponding CMA3-positive sites shown by thick arrows (in c and d) and shown in inset (in d), other CMA3–positive sites shown by thin arrows (in d).
