Table 1.
Electron transport chain components and energy generating machinery of mycobacteria
| Operon/ Gene1 |
Subunits | Rv # | Enzyme Name | In vitro Essentiality | |
|---|---|---|---|---|---|
| Griffin et al2 | Zhang et al3 | ||||
| nuo | nuoABCDEFGHIJKLMN | Rv3145–Rv3158 | Type I NADH:Menaquinone oxidoreductase | nuoDFGHI = yes | nuoDFGL = yes |
| ndh | - | Rv1854c | Type II NADH:Menaquinone oxidoreductase | yes | no |
| ndhA | - | Rv0392c | Type II NADH:Menaquinone oxidoreductase | no | no |
| sdh1 |
sdh1CD sdh1A sdh1B |
Rv0249c Rv0248c Rv0247c |
Succinate:Menaquinone oxidoreductase I | yes | Only sdh1A |
| sdh2 |
sdh2C sdh2D sdh2A sdh2B |
Rv3316 Rv3317 Rv3318 Rv3319 |
Succinate:Menaquinone oxidoreductase II | no | no |
| mqo | - | Rv2852c | Malate:Menaquinone oxidoreductase | no | no |
| pru |
pruA pruB |
Rv1187 Rv1188 |
Proline dehydrogenase and pyrroline-5-carboxylate dehydrogenase | yes | yes |
| gpdA1 | - | Rv0564c | Glycerol-3-phosphase dehydrogenase A1 | no | no |
| gpdA2 | - | Rv2982c | Glycerol-3-phosphase dehydrogenase A2 | no | no |
| glpD1 | - - |
Rv2249c (Rv2250c) |
Glycerol-3-phosphase dehydrogenase D1 | no | yes |
| glpD2 |
glpD2 ldpA |
Rv3302c Rv3303c |
Glycerol-3-phosphase dehydrogenase D2 Dihydrolipoamide dehydrogenase |
yes | yes |
| cox | coxCMSLDEFG | Rv0376c–Rv0368c | Carbon monoxide dehydrogenase | coxLEF = yes | coxCL = yes |
| ald | - | Rv2780 | L-Alanine dehydrogenase | no | no |
| lldD1 |
? pqqE lldD1 |
Rv0692 Rv0693 Rv0694 |
L-Lactate dehydrogenase 1 | no | lldD1 = yes |
| lldD2 |
lldD2 - - |
Rv1872c Rv1871c Rv1870c |
L-Lactate dehydrogenase 2 | no | no |
| Rv0843 | - | (Rv0842) Rv0843 |
Pyruvate dehydrogenase E1 component alpha subunit | no | no |
| pdh |
pdhA pdhB pdhC |
Rv2497c Rv2496c Rv2495c |
Pyruvate dehydrogenase | pdhB = yes | pdhC =yes |
| ace | - | Rv2241 | Pyruvate dehydrogenase E1 component | yes | yes |
| lpd | - | Rv0462 | Dihydrolipoamide dehydrogenase | yes | yes |
| hyc | Rv0081 Rv0082 Rv0083 hycDPQE Rv0088 |
Rv0081-Rv0088 | Formate hydrogenlyase OR Energy-converting hydrogenase related complex (Ehr) (a.k.a HydTB) | hycE and Rv0088 = yes | hycQE = yes |
| qcr |
qcrC qcrA qcrB |
Rv2194 Rv2195 Rv2196 |
Cytochrome bc1 | yes | yes |
| ctaB | - | Rv1451 | Cytochrome c oxidase assembly factor | yes | yes |
| ctaC |
ctaC - |
Rv2200c (Rv2199c) |
Transmembrane Cytochrome C subunit II | yes | Rv2200c = yes |
| ctaD | - | Rv3043c | Cytochrome C oxidase polypeptide I | yes | yes |
| ctaE | - | Rv2193 | Cytochrome C oxidase subunit III | yes | yes |
| cyd |
cydA cydB cydD cydC |
Rv1623c Rv1622c Rv1621c Rv1620c |
Cytochrome bd oxidase | yes | cydBC = yes |
| nar |
narG narH narJ narI |
Rv1161 Rv1162 Rv1163 Rv1164 |
Menaquinone:Nitrate reductase | no | no |
| sirA |
sirA cysH che1 |
Rv2391 Rv2392 Rv2393 |
Sulfite reductase APS reductase ferrochelatase |
yes | Rv2391 and Rv2392 = yes |
| nirBD |
nirB nirD |
Rv0252 Rv0253 |
Nitrite reductase [NAD(P)H] | no | no |
| frd |
frdA frdB frdC frdD |
Rv1552 Rv1553 Rv1554 Rv1555 |
Menaquinone:Fumarate reductase | no | frdA = yes |
| atp | atpIBEFHAGDC and Rv1312 | Rv1303-Rv1312 | F1Fo ATP synthase operon | yes | yes except atpI and Rv1312 |
1
In the absence of operons confirmed by previous publications, the TB Database operon prediction algorithms (www.tbdb.org) were used to identify potential operons. Rv loci enclosed in parentheses signify weak operon predictions.
2
Griffin et al. (26)
3
Zhang et al. (129). N.B. Genes identified as containing both essential and non-essential regions (a “D call”) are referred to as essential here.