Figure 3.
Tat transcriptionally modulates Nav1.7 and Nav1.8. A, Representative raw traces of voltage-clamp experiments showing the presence of TTX-sensitive and -resistant sodium channels in neurons isolated from the adult mouse ileum. B, C, Quantitative PCR of LMMP preparations after 30 min of Tat treatment. Nav1.7 and Nav1.8 mRNA levels are unchanged. D, After long-term exposure (2–3 d) to Tat, Nav1.7 and Nav1.8 mRNA levels are significantly increased, but Nav1.3 and Nav1.9 levels are not affected by Tat pretreatment. E, Significantly higher Nav1.7 and Nav1.8 mRNA are observed in Tat+ compared with Tat− LMMPs. *p < 0.05 (ANOVA followed by Bonferroni's post hoc test).