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. 2014 Oct 22;34(43):14304–14317. doi: 10.1523/JNEUROSCI.0930-14.2014

Figure 6.

Figure 6.

Loss of Drp1 disrupts mitochondrial morphology and decreases mitochondrial mass in DA neurons. A–F, Mitochondrial morphology and mass in midbrain DA neurons from 1-month-old Drp1KO and control mice. A, Loss of Drp1 causes mitochondria to become visibly swollen in most midbrain DA neurons (identified by TH staining, red). B, Mitochondrial mass decreased upon determining the mean fluorescence (normalized to cell area) of neurons stained with Tom20 (C), AAVmitoGFP (AFU, arbitrary fluorescent units) (D), PDH (E), and cytochrome c oxidase I (Cox I; F). Scale bars: 5 μm. Data show mean ± SEM, *p < 0.05, **p < 0.001, ***p < 0.001 by unpaired two-tailed t test, n = 3–4 mice per group, 36–76 cells quantified per mouse. G, H, Ultrastructural analysis at the cell body by immunogold staining against TH revealed larger mitochondria in Drp1KO mice than in controls. Scale bars: 1 μm. Data show mean ± SEM, n = 3 mice per group, 11–16 cell bodies quantified per mouse.