Figure 8.

Blocking ACAT1 did not induce ER stress in microglia. A, N9 cells were treated with 0.5 μm K604, or with 2.5 μg/ml tunicamycin, or with 1 μm thapsigargin for 8 h. Total RNA was isolated, and cDNA was synthesized using reverse transcriptase. Expression levels of UPR-target genes were analyzed by qPCR. Data are mean ± SEM of two experiments. **p < 0.01. ***p < 0.001. n.s., Not significant. B, Expression levels of UPR-target genes were determined by qPCR. cDNA was synthesized from total RNA obtained from primary microglia. Data are mean ± SEM of three experiments. n.s., Not significant. C, Unspliced form (uXBP1) and spliced form (sXBP1) of XBP1 were detected by RT-PCR. Tm, tunicamycin; Tg, thapsigargin.