(A) Distance of the MTOC in NK cells from the site of contact with S2 or S2–ICAM-1 cells, relative to the cell diameter, after silencing of the indicated genes. Each dot represents a measurement from a single cell. The graph shows the median from measurements collected in 3 experiments. N. s., not significant; *p<0.05; **p<0.01. (B) Polarization of the MTOC toward S2 and S2–ICAM-1 cells and granule convergence to the MTOC, as indicated, were scored in the absence or presence of an anti-S2 rabbit serum. A minimum of 34 conjugates were analyzed.