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. Author manuscript; available in PMC: 2014 Oct 22.
Published in final edited form as: Mol Endocrinol. 1994 Feb;8(2):240–248. doi: 10.1210/mend.8.2.8170480

Fig. 10. Colocalization of Phospho-CREB Induction with the Transgene in PVN.

Fig. 10

Low power sections through the PVN from transgenic mice killed 10 min after ip injection of 0.15 M saline (A) or 1.5 M saline (B). C shows high power oil immersion photomicrographs of cells stained for β-galactosidase and phospho-CREB imrnunoreactivities. The animals were handled daily for 5 days before the experiment to reduce the known effects of handling on induction of immediate early genes in the PVN. Minimal induction (as assessed by intensity of neuronal staining) is present in the PVN in both the control and 0.15 M saline groups compared with that in the stressed animals. Phospho-CREB is rapidly activated and may reflect the underlying mechanism by which CREB-like proteins bind to the regulatory sites within the proenkephalin gene, second messenger-inducible enhancer. Magnification: horizontal bars = 50 μm (A and B) and 10 μm(C).