Each lane represents extracts made from the PVN regions pooled from three animals. For each oligonucleotide, the first lane represents no treatment, the next two lanes represent 1.5 M saline injection, and the last two lanes represent 0.15 M saline injection. Animals were killed 2 h after injection. Each saline condition is represented by two lanes, each representing an independent experiment. The arrows point to a specific band, as determined by competition analysis (see Fig. 7). Top, AP-1 oligonucleotide. Middle, CRE-2 oligonucleotide. Bottom, CaRE oligonucleotide.