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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Arthritis Rheumatol. 2014 Jun;66(6):1504–1516. doi: 10.1002/art.38402

Figure 6.

Figure 6

Plasminogen deficiency results in diminished matrix metalloproteinase 9 (MMP-9), but not MMP-2, activity in the knees but not the paws of Tg197 mice. A–D, Results of quantitative reverse transcriptase–polymerase chain reaction analysis of MMP-9 and MMP-2 mRNA isolated from the paws (A and B) and knees (C and D) of 10-week-old Plg+ and Plg− Tg197 mice. Bars show the mean ± SEM. P values were determined by Student’s t-test. E and F, Gelatin zymography of protein extracts prepared from the paws (E) and knees (F) of Tg197 mice to detect MMP-9 and MMP-2 activity. G, Western blot analysis using an anti–MMP-9 antibody, which detects both proMMP-9 (105 kd) and active MMP-9 (act MMP-9; 88 kd). Note that active MMP-9 is readily detected in the knees of Plg+/Tg197+ mice, but that levels of active MMP-9 are significantly reduced in the knees of Plg−/Tg197+ mice, despite higher overall levels of proMMP-9 protein. NS = not significant.