Fig. 2.

Time course of CREB phosphorylation after stimulation with forskolin in primary striatal cultures. Primary striatal cultures were treated with forskolin (20 μM) for the times indicated, medium was removed and cells were frozen in liquid nitrogen until the day of the experiment. The immunoblot was developed with ¹³³Ser-phos-pho-CREB antibody (upper blot), and reprobed with CREB antiserum (lower blot). The specific bands ran slightly above the 43-kD marker. No significant difference of ¹³³Ser-phospho-CREB induction was observed between 5 min and 3 h. No significant differences of CREB levels were observed at any time points (n = 2).