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. Author manuscript; available in PMC: 2014 Oct 22.
Published in final edited form as: J Neurochem. 2003 Oct;87(1):107–118. doi: 10.1046/j.1471-4159.2003.01992.x

Fig. 4.

Fig. 4

CREB phosphorylation is super-induced by the D1 agonist SKF 82958 during naloxone-precipitated withdrawal from chronic morphine. Primary striatal cultures were treated with either vehicle or morphine (morph, 10 μM) for (a) 1.5 h (acute) or (b) 6 days (chronic) followed by 30 min incubation with vehicle or naloxone (nal, 10 μM) and 15 min incubation with vehicle or SKF 82958 (SKF, 50 μM). The ratio of P-CREB/actin was determined for each sample and normalized to the control group ratio to yield a fold induction. Data are plotted as the mean fold induction ± SEM. **p < 0.01 compared with control. ††p < 0.01 compared with the chronic morphine plus naloxone plus SKF 82958 treatment group. ⟡⟡p < 0.05 between acute vehicle plus SKF 82958 and acute morphine plus SKF 82958. N = 4 experiments with treatments in triplicate (a,b). Representative blots are shown.