Fig. 4.
Function and location of low affinity activated T cells. (A) Splenic sections were stained for B220 (red) to identify B-cell zones, F4/80 (blue) to identify red pulp area, and CD45.2 (green) to identify B3K508 donor T cells. Percentage of B3K508 T cells located in the indicated areas at day 6. (B) Th1 and TFH cells were sorted from infected mice and transferred into CD3ε−/− mice immunized 1 d earlier with 3K-OVA and alum. GC B-cell numbers correlate with TFH cell numbers. (C) Intracellular cytokine production was assessed by flow cytometry. Data represent n ≥ 3 mice per group and at least two independent experiments.