Fig. 3.

Prevalence of slowly inactivating MA current in duck TG neurons. (A) Exemplar traces showing MA current from TG neurons recorded in the whole-cell configuration in the voltage-clamp mode. Mechanical stimulation was delivered by a glass probe at 800 µm/s velocity. (Inset) A duck TG neuron with the electrode and the probe (black and gray arrowheads, respectively) in the working positions. (Scale bar, 20 µm.) (B) Exemplar traces of mouse and duck TG neurons with fast, intermediate, and slow kinetics of inactivation. The τ-values were obtained by fitting the inactivating current component to a monoexponential equation. (C) Quantification of TG neurons with different inactivation kinetics. Of all mouse (n = 47) and duck (n = 84) TG neurons, 87 and 79% were mechanosensitive. (D) Histogram of TG neurons based on the τ-values; dotted lines represent median. (E) Quantification of the peak MA current amplitude in TG neurons in response to a 6 µm deep mechanical stimulation. *P < 0.05, **P < 0.01, ***P < 0.001, nonparametric Mann–Whitney U test.