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. 2014 Sep 30;111(41):14906–14911. doi: 10.1073/pnas.1415825111

Fig. 1.

Fig. 1.

Age-related changes of adipose vasculature and scWAT adipocyte size. (A) Body weight and BMI of 1-, 4-, 10-, 12-, and 16-mo-old mice (n = 10–12). (B) Histology of adipocytes and vascular density of scWAT. scWATs were stained with H&E, CD31 (red), or perilipin (PLPN, green) plus isolectin B4 (I-B4, red) counterstained with DAPI (blue) nucleus staining. Arrowheads point to microvessels. Dashed lines show diameters of typical adipocytes. (Scale bars, 50 μm.) (C) Average diameters of adipocytes of scWAT. Adipocyte sizes were quantified from 120 adipocytes from 12 fields. (D) Quantification of total microvessel density in scWAT (12 fields per group). (E) Quantification of microvessel numbers per adipocyte in scWAT (total numbers of microvessels were divided by total numbers of adipocytes per field; 12 fields per group). *P < 0.05, ***P < 0.001.