Fig. 1.

The absence of Tsc1 diminishes the generation of memory T cells. (A) Flow cytometry of the frequency of K^b-OVA⁺ CD8⁺ T cells in the blood from WT and Tsc1^−/− mice infected with LM–OVA at the indicated time points (*P < 0.05, **P < 0.01). (B) Number of K^b-OVA⁺ CD8⁺ T cells in the spleen from WT and Tsc1^−/− mice at days 0, 9, 18, and 41 p.i. (C) Flow cytometry of CD8⁺ T cells (Left) and the number of K^b-OVA⁺ CD8⁺ T cells (Right) in the spleen and liver from WT and Tsc1^−/− mice at day 41 p.i. (D) Splenocytes from WT and Tsc1^−/− mice at day 41 p.i. were restimulated with the SIINFEKL peptide for intracellular cytokine staining of IFN-γ and TNF-α. Shown are representative flow cytometry plots of CD8⁺ T cells (Left) and the number (Right) of IFN-γ⁺ and TNF-α⁺ CD8⁺ T cells. Data are representative of three independent experiments and are presented as the mean ± SEM.