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. 2014 Sep 30;111(41):14858–14863. doi: 10.1073/pnas.1404264111

Fig. 3.

Fig. 3.

Tsc1 promotes memory T-cell generation through cell-intrinsic mechanisms. Naïve CD8+ T cells from OT-I+ (CD45.2+) and Tsc1−/−OT-I+ (CD45.1.2+) mice were isolated, mixed at a 1:1 ratio, and transferred to CD45.1+ recipients, followed by LM–OVA infection 1 d later. (A) Flow cytometry and frequencies of OT-I+ (CD45.2+) and Tsc1−/−OT-I+ (CD45.1.2+) donor T cells out of the total Kb-OVA+ CD8+ cells in the blood at days 9, 29, and 57 p.i. (B–D) At day 57 p.i., mice were rechallenged with LM–OVA and analyzed 3 d later by flow cytometry. (Right) The frequencies and numbers of OT-I+ (CD45.2+) and Tsc1−/−OT-I+ (CD45.1.2+) out of the total Kb-OVA+ CD8+ T cells from the blood, spleen, and liver (B) or out of splenic IFN-γ+ (C) or TNF-α+ (D) CD8+ T cells after restimulation with SIINFEKL peptide. Data are representative of three independent experiments and are presented as the mean ± SEM.