Fig. 5.

Tsc1-dependent gene expression profiles in antigen-experienced CD8⁺ T cells. (A) Scatterplot comparison of global gene expression profiles between WT and Tsc1^−/− K^b-OVA⁺ CD8⁺ T cells sorted from mice at day 9 p.i.; transcripts with >0.5 log₂ fold change are highlighted (WT, n = 4; Tsc1^−/−, n = 5). (B) Heat maps of differentially expressed genes in K^b-OVA⁺ CD8⁺ T cells (with >0.5 log₂ fold change), with red color denoting up-regulated genes in Tsc1^−/− cells and blue color down-regulated genes in Tsc1^−/− cells. (C) Comparison of expression changes in Tsc1^−/− versus WT cells with those in KLRG1^hi versus KLRG1^lo cells. The Tsc1 target genes (>0.5 log₂ fold change) were partitioned into four main clusters, shown and colored by regions (R1–R4). Numbers within parentheses indicate the number of probes within each subregion. (D and E) ROS production (D) and cell size (E) of splenic K^b-OVA⁺ CD8⁺ T cells from WT and Tsc1^−/− mice at day 9 and 18 p.i. Data are representative of one experiment (A–C) and two independent experiments (D and E) and are presented as the mean ± SEM.