Table 2.
DAVID functional pathway analysis of up-regulated VitD3-sensitive genes
| Biological pathway | No. of genes | Gene description and symbol | DAVID P value | |
| Up-regulated by high dietary VitD3 | ||||
| G protein-coupled receptor activity | 5 | Dopamine receptor 5 (Drd5); glutamate receptor, ionotropic, kainate 3 (Grik3); neuromedin B receptor (Nmbr)*; serotonin receptor 2C (Htr2c), thyroid-stimulating hormone releasing hormone receptor (Trhr) | 0.004 | |
| Synaptic transmission | 4 | Synaptojanin 1 (Synj1)*, synaptotagmin 2 (Syt2), Drd5, Grik3 | 0.03 | |
| Cell communication | 5 | Nuclear receptor 4A2 (Nr4a2)*, Drd5, Grik3, Synj1*, Syt2 | 0.05 | |
| Up-regulated by medium and high dietary VitD3 (dose-dependent) | ||||
| Cation homeostasis | 4 | Plasma membrane Ca2+ ATPase 4 (Atp2b4)*, Ca2+/calmodulin-dependent protein kinase IIδ (Camk2d)*, Na+/H+ exchanger 7 (Slc9a7), synaptophysin-like 2 (Sypl2) | 0.003 | |
| Cytoplasmic vesicle membrane part | 5 | Vesicular glutamate transporter 2 (Slc17a6)*, Atp2b4*, Camk2d*, Slc9a7, Sypl2 | 0.03 | |
| Calcium ion binding | 4 | Delta/notch-like EGF repeat containing (Dner)*; neurocalcin-delta (Ncald); sparc/osteonectin, cwcv, and kazal-like 3 (Spock3)*; Atb2b4* | 0.05 | |
Specific functional pathways altered in the hippocampus by dietary VitD3 dose are indicated underneath the bold headings.
*
Highly significant genes identified in microarrays.