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. 2014 Oct 1;111(41):14800–14805. doi: 10.1073/pnas.1409588111

Fig. 2.

Fig. 2.

The Hac1-independent ER stress response has the hallmarks of PKA deactivation. (A) Enrichment of the STRE (Msn2/4 binding site) motif in the group of 280 Hac1-independent genes targets. The HSE (Hsf1 binding site) was not enriched. (B) Enrichment of the RRPE (Stb3 binding site) and PAC (Dot6/Tod6 binding site) motifs. (C) Fold change of gene expression with 100 nM estradiol (Est) vs. 5 µg/mL tunicamycin (Tm). Msn2/4 targets are highlighted in red; genes annotated as ribosomal protein genes or genes involved in ribosome biogenesis are highlighted in blue. (D) Relative PKA activity in cell extracts from a tpk1/2/3-as strain treated with 1NM-PP1 or a wild-type strain treated with 5 µg/mL tunicamycin for 4h.