Table 2.

T-cell clones respond to register-fixed DQ8/Ins complexes in R3

DQ8-Ins	Peptide*	T1D#10 C8†	T1D#3 C8	T1D#4 C5
I72C-R1	LEEALYLVAEEC	—	—	—
I72C-R2	VEALYLVAGERC	—	—	—
I72C-R3	VEELYLVAGEEGC	1.5	31.6	4.9
I72C-R4	VEAEYLVAGEREGC	—	0.4	0.1
V65C-R1	LEEALYCVAEER	—	—	—
V65C-R2	VEALYLCAGERG	—	—	—
V65C-R3	VEELYLVCGEEGG	2.3	38.8	0.4
V65C-R4	VEAEYLVACEREGG	—	—	—

The lowest concentration of IFN-γ standard was 0.017 ng/mL and readings below this level are indicated as with an “—.”

^*To “trap” each peptide in a specific register, corresponding p1 and p9 anchor residues of the peptide were mutated to E (in bold). To “fix” the peptide in a specific register by disulfide bonding, each set of peptides had a single C residue (underlined) at position p6 (to bind to V65C) or at p11 (to bind to I72C). The other C residue that would not be at p6 was changed to A (Italic).

^†T-cell clones were stimulated with B6K10 cells expressing the indicated register-fixed constructs and assayed for secreted human IFN-γ (ng/mL).