Fig. 3.

Altered expression of CA9 following demethylation in SK-OV-3 cells. SK-OV-3 cells were treated for three days with 0, 5, or 10 µM 5-aza-2'-deoxycytidine, respectively. After treatment with 5-aza-2'-deoxycytidine, CA9 mRNA expression was measured by qRT-PCR (A). The DNA methylation status at the specific CpG site was analyzed by quantitative MSP (B). Data are shown as the means±SDs (n=3). Statistical analyses were performed with one-way ANOVA and subsequent Bonferroni tests (^*p<0.05). M, the percentage of methylated CpGs; U, the percentage of unmethylated CpGs; qRT-PCR, quantitative reverse-transcription polymerase chain reaction; CA9, carbonic anhydrase 9; MSP, methylation-specific PCR; ANOVA, analysis of variance.