Fig. 4.

CA9 promotes migration of SK-OV-3 cells. Migration of serum-starved cells towards 15% serum-containing media was examined in a transwell assay. Cells that migrated through an 8-µm pore filter were fixed and stained with crystal violet. Representative images of migrated cells transfected with EGFP or CA9 are shown (A). Quantitative analysis of migrated cells was performed by measuring the absorbance of extracts from cell stains at 595 nm. Data are shown as means±SDs for triplicate measurements (B). Statistical analysis was performed with a t-test (^*p<0.05). CA9, carbonic anhydrase 9; EGFP, enhanced green fluorescent protein.