Fig. 5.

CA9 enhances the invasiveness of SK-OV-3 cells. Invasion of serum-starved cells towards 10% serum-containing media was examined in a Matrigel-coated invasion chamber. The cells that invaded through Matrigel were fixed and stained with crystal violet. Representative images of invading cells transfected with EGFP or CA9 are shown (A). Quantitative analysis of invading cells was performed by measuring the absorbance of extracts from cell stains at 595 nm. Data are shown as means±SDs for triplicate measurements (B). Statistical analysis was performed with a t-test (^*p<0.05). CA9, carbonic anhydrase 9; EGFP, enhanced green fluorescent protein.