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Western analysis in native, vector-only, and LOXL1 over-expressing, stable transfected HEK293 cells. A: E-cadherin and LOXL1 immunoblot. LOXL1 full length (arrowhead) and processed (arrows) forms. B: LOXL1 over-expression reduced E-cadherin levels. Relative expression of E-cadherin was normalized to GAPDH loading control. C.Immuno-precipitated Snail1 and LOXL1 were probed for LOXL1 and Snail1 respectively. Snail1 expression was detected in all three cell lines.
