Figure 5.

Astro- and microgliosis. GFAP-staining in the perilesional area (same area as in Figure 4E), at 24 h (A) and 7 days (B) post-injury, both with 100-bar loading-pressure. (C) Graph showing the staining intensity at 24, 72 h, and 7 days post-injury for 35-, 50-, and 100-bar loading-pressure. Data expressed as mean ± SD (n = 5 in each group, ***p < 0.001 ANOVA followed by Bonferroni’s test. Iba1-staining (D,E) after 100-bar loading-pressure. (F) Graph of staining intensity at 24, 72 h, and 7 days post-injury for 35-, 50-, and 100-bar loading-pressure. Data expressed as mean ± SD (n = 5 in each group). *p < 0.05, **p < 0.01, ***p < 0.001 (ANOVA followed by Bonferroni’s test). Scale bar = 50 μm