FIGURE 5.

Quantitative analysis of dendritic spine morphological changes in BPA exposed mice. (A) A representative high magnification confocal image of dendritic spines on apical dendrites of layer 5 neurons. Lines indicated measurements of dendritic spine parameters. Spine Length measurements (tip of the spine to its base at the dendrite, red line); Spine head diameter (measured at the widest point of spine, blue line) and relative neck width (blue line across neck). Scale bar = 2.5 μm. (B) Morphological measurements of dendritic spines, including spine head diameter, spine length and relative neck diameter, were similar for oil treated (clear bar) vs. BPA treated (solid bar) mice. (C) Spines can be loosely classified into morphological “types” by comparing their relative head, neck, and length dimensions. The complete overlap of spines from the two conditions indicates that BPA exposure does not elicit a change in spine type distribution (oil = filled in circle, BPA = open square).