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. 2014 Oct 22;4:6644. doi: 10.1038/srep06644

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DNA encoding the cap of porcine-derived AAVs was transfected into HEK293T cells. Cell lysate was harvested and western blot performed using monoclonal antibody against AAV VP1, VP2, and VP3 (Fitzgerald). Western blot reveals that porcine-derived AAVs form VP1, VP2, and VP3 with sizes of ~87, 72, and 62 kiloDaltons, respectively. AAV5 VP1, VP2, and VP3 were used as a comparison.