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. 2014 Oct 22;5:550. doi: 10.3389/fmicb.2014.00550

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Copyright © 2014 Lima, Muniz and Dumont.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Methanotroph pmoA abundances in Amazonian Dark Earth and adjacent soils under secondary forest and manioc cultivation at Barro Branco and Caldeirão sites. (A) Abundance of pmoA genes using the A189f-mb661r qPCR assay targeting conventional pmoA genes (i.e., mostly Methylocystis in these soils). (B) Abundance of USCα pmoA genes determined with the A189f-Forest675r qPCR assay. Abundances denoted with different letters above the bars are significantly different from each other (Tukey’s HSD, p < 0.05). ND indicates that the target gene was below the detection limit of the qPCR assay, which is indicated by the dashed line.