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. 2014 Oct;2(10):97. doi: 10.3978/j.issn.2305-5839.2014.09.05

Figure 4.

Figure 4

Growth characteristics of MEF and HFF cells grown in Neuro-Pure. (A) Cells were grown in Neuro-Pure under various condition; normal without additional factors (NX), unrefrigerated for 2 weeks (NX-U), with serum (NX-+) and in the recommend media with serum (SM). Cell were grown for 24 hrs and an MTT assay was conducted; (B) the same cells mentioned in (A), were grown for 10 passages under normal conditions at the initial plating density (0 hrs) and the density at 48 hours grown in Neuro-Pure (48 hrs) and at 48 hours in conventional serum medium as a control (Cont). Cells were counted manually using trypan blue and a hemocytometer; (C) images of cells taken at 20× to show morphology of cells are typical for their respective lineage. Cell counts were taken from n=3 independent experiments with a (*P≤0.05) versus Neuro-Pure [(A) 24 hrs and (B) 0 hrs], while (**P≤0.05) versus Neuro-Pure at the time point of 48 hrs (B).