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HIF-1α-siRNA suppressed HIF-1α and VEGF expression. (A) mRNA expression of HIF-1α and VEGF was suppressed by HIF-1α-siRNA in Cal-27 cells; (B) protein expression of HIF-1α and VEGF was suppressed by HIF-1α-siRNA in Cal-27 cells at 48 h. The right panel is quantification of Western blotting results. Data were expressed as mean ± SD (n=5). HIF-1α, hypoxia-inducible factor-1 alpha; VEGF, vascular endothelial growth factor.
