Figure 6. Functions of Dax1 and Nanog are partially complementary but they cannot replace each other.

(a) qRT–PCR to measure expression of pluripotency and ExEn markers in the indicated lines after 5 days culture with LIF. All data are normalized to Gapdh and shown relative to WT ESCs (set at 1.0). Data are means±s.d.; n=3. (b) Immunofluorescence analysis of Gata6 in the indicated lines. Monolayer cultures were treated with RA (0.1 μM) for 4 days and counterstained with DAPI. Scale bar, 100 μm.