Figure 4.

The use of particle filtration and dilution culturing in 48-well plates for the isolation of marine fungi. (a) Schematic of the particle filtration and dilution plating workflow. (b) Algal particles that were retained on filter sieve after homogenization and filtration. (c) Plating into 48-well plates allows for the separation of fast and slower growing isolates, increasing the isolation frequency of slower-growing fungi. (d) Conidia from a strain of marine fungus Anguillospora marina (telemorph = Lindra obtusa) isolated from algal tissue particles (scale bar = 20 µm). (e) Conidia and conidiophores of a strain of the marine fungus Paradendryphiella arenaria isolated from algal tissue particles (scale bar = 20 µm).