Table 2. Hydrolysis conditions used in assays for pectinase activities reported in the literature.
Microorganism (cultivation mode)* | Pectic substrate & concentration (% m/v)# | T (°C) | t (min) | pH | Activity (SSF: U/g; SLF: U/mL) | Reference |
Thermomucor indicae-seudaticae (SSF) | 1% pectin | 60 | 10 | 5.5 | 108 | [20] |
Penicillium viridicatum (SSF) | 0.8% pectin | 50 | 10 | 5.5 | 71 | [21] |
Thermoascus aurantiacus (SSF) | 0.8% pectin | 55 | 10 | 5.0 | 43 | [22] |
Fusarium moniliforme (SSF) | 0.5% pectin | 40 | 30 | 4.5 | 43 | [23] |
Aspergillus niger (SSF) | 0.45% pectin | 45 | 30 | 4.5 | 25 | [24] |
Aspergillus awamori (SSF) | 0.33% pectin | 45 | 10 | 5.0 | 9 | [25] |
Streptomyces sp. (SLF) | 1% pectin | 60 | - | 3 | 162 | [26] |
Penicillium occitanis (SLF) | 0.45% pectin | 50 | 60 | 4.8 | 64 | [27] |
Aspergillus niger (SLF) | 0.5% pectin | - | 15 | 4.5 | 34 | [28] |
Aspergillus flavipes (SLF) | 1% pectin | 45 | 20 | 5 | 20 | [29] |
Aspergillus niger (SLF) | 0.2% pectin | 30 | - | 5.3 | 1.3 | [30] |
Trichoderma viridae (SlSF) | 0.2% PGA | 30 | - | 5 | 10 | [31] |
Aspergillus sojae (SSF) | 0.2% PGA | 26 | - | 6.6 | 30 | [32] |
Bacillus subtilis (SSF) | 0.24% PGA | 65 | 10 | 9.5 | 6592 | [33] |
Aspergillus niger (SSF) | - | 40 | - | 3.5 | 135 | [34] |
Aspergillus sp. (SSF) | 0.17% PGA | - | 10 | 5.3 | 390 | [35] |
Penicillium grisereoserum (SLF) | 0.75% PGA | 40 | 20 | 4.8 | 3490 | [36] |
*SSF: solid-state fermentation; SLF: submerged fermentation; SlSF: slurry-state fermentation.
PGA: polygalacturonic acid.
-: not specified.