Table 2. Primer used for hepatitis B virus DNA amplification.
| Target | Primers, 5′-3′ | Product size (bp) | Function |
| HBV cccDNA CpG island I, F | TTTGTTGGTGGTTTTAGTTTAGGA | Forward primer for 1st and 2nd round cccDNA CpG island I | |
| HBV cccDNA CpG island I, R | AACAACATACCTTAATAATCCAA | 413 | Reverse primer for 1st round cccDNA CpG island I |
| HBV cccDNA CpG island I, R2 | TCCCCCTAAAAAATTAAAAAAAATC | 231 | Reverse primer for 2nd round cccDNA CpG island I |
| HBV cccDNA CpG island II, F | TTTTATGGTTGTTAGGDTGTGTTGTT | Forward primer for 1st round cccDNA CpG island II | |
| HBV cccDNA CpG island II, R | TAACCTAAHCTCCTCCCCCAAC | 391 | Reverse primer for 1st round cccDNA CpG island II |
| HBV cccDNA CpG island II, F2 | TGTGTTGTTAATTGGATTTTG | Forward primer for 2nd round cccDNA CpG island II | |
| HBV cccDNA CpG island II, R2 | ATCCTCTTATAYAAAACCTTAAACAA | 277 | Reverse primer for 2nd round cccDNA CpG island II |
| HBV cccDNA CpG island III, F | GTTATGTTAATGTTAATATGGGT | 299 | Forward primer for 1st round cccDNA CpG island III |
| HBV cccDNA CpG island III, F2 | GTGGTTTTATATTTTTTGTTTTAT | 256 | Forward primer for 2nd round cccDNA CpG island III |
| HBV cccDNA CpG island III, R | AAAATACTAACATTAAAATTCCCAAA | Reverse primer for 1st and 2nd round cccDNA CpG island III | |
| S1 | GTCACCATATTCTTGGGAAC | 1286 | Amplify S gene to determine the HBV genotype |
| AS | CATATCCCATGAAGTTAAGG | Amplify S gene to determine the HBV genotype | |
| CpG island I in vitro, F | AACAAGATCTACAGCATGGGAGGT | 1493 | Forward primer for CpG island I containing fragment |
| CpG island I in vitro, R | GTAAGTTGGCGAGAAAATAAAAGC | Reverse primer for CpG island I containing fragment | |
| CpG island I complement in vitro, F | ATTGTGGGTCTTTTGGGGTTT | 1972 | Forward primer for CpG island I remaining HBV DNA fragment |
| CpG island I complement in vitro, R | GATTTTCTGAGTTGGCTTTGA | Reverse primer for CpG island I remaining HBV DNA fragment | |
| CpG island II in vitro, F | CCGCCCCTTTCACGCAATGTGGATA | 840 | Forward primer for CpG island II containing fragment |
| CpG island II in vitro, R | GGATCCCCGGGTACCGAGCTCTTCA | Reverse primer for CpG island II containing fragment | |
| CpG island II complement in vitro, F | GTTGTAAAACGACGGCCAGTG | 2537 | Forward primer for CpG island II remaining HBV DNA fragment |
| CpG island II complement in vitro, R | AGTTGGCGAGAAAATAAAAGC | Reverse primer for CpG island II remaining HBV DNA fragment | |
| CpG island III in vitro, F | AAGTTGGGTAACGCCAGGGTTTTCC | 1164 | Forward primer for CpG island III containing fragment |
| CpG island III in vitro, R | CCAGGGGATTGGGGACAGAAAGATT | Reverse primer for CpG island III containing fragment | |
| CpG island III complement in vitro, F | CGCCTCATTTTGCGGGTCACCATAT | 2269 | Forward primer for CpG island III remaining HBV DNA fragment |
| CpG island III complement in vitro, R | CTAGAGGATCCCCGGGTACCGAGCT | Reverse primer for CpG island III remaining HBV DNA fragment |
Degenerate base: D (A, T); H (A, T, C); Y (C, T).