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. 2014 Oct 22;9(10):e110269. doi: 10.1371/journal.pone.0110269

Figure 3. IR-induced Changes in Signaling Pathways 1, 3 and 10 months post-IR.

Figure 3

Representative western blot scans of heart tissue homogenates from sham controls, 1H- and 56Fe-IR mice at 1, 3 and 10 months post-IR in IR+Aging model. Bands represent phosphorylated (p), total (T) and loading control for the following proteins - NCX and GAPDH (A, I, Q), SERCA2a and GAPDH (C, K, S), p-NFATc4, T-NFATc4 and β-actinin (E, M, U), p-p38, T-p38 and GAPDH (G, O, W). Quantification and graphic representation of total protein levels and phosphorylation using densitometric analysis of phospho-band intensities after adjusting for corresponding GAPDH/β-actinin and total band intensities of heart tissue homogenates at 1, 3 and 10 months post-IR for the following proteins - NCX (B, J, R), SERCA2a (D, L, T), p-NFATc4 (F, N, V), p-p38 (H and P). Results in all graphs represent mean ± SEM of the pooled data from n = 6–8 animals per time point/group for non-IR control (solid black bars), 1H-IR (solid grey bars) and 56Fe-IR (solid white bars) groups. Statistical significance was assigned when P<0.05.