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. 2014 Oct 22;9(10):e110653. doi: 10.1371/journal.pone.0110653

Figure 1. Skeletal muscle inflammation is increased in ob/ob mice.

Figure 1

Epidydimal fat (eWAT), quadriceps (quads) and gastrocnemius (gastrocs) of wild –type (wt) and ob/ob mice were collected prior to analysis with qPCR of CD68 (A), CD11c (B), TNFα (C) and MCP1 D) qPCR analysis was performed for F4/80, IL1β, RANTES, TLR4, Foxp3, CD4 and CD8a in quadriceps from wt and ob/ob mice. E) qPCR analysis was performed for CX3CL1, CX3CR1 and IL10 in wt and ob/ob mice quadriceps. F–G) TNFα (E) and MCP1 (F) protein concentration in gastrocnemius of wt and ob/ob mice were quantified by ELISA. * stands for P<0.05 and ** for P<0.01; n = 5.