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. 2014 Oct 22;9(10):e110916. doi: 10.1371/journal.pone.0110916

Figure 5. Tumorigenicity assay of ZYM-SIEC02 cells.

Figure 5

A. Representative images of pSIECs and ZYM-SIEC02 cells growing in soft agar. Neither cell line formed colonies after 2 weeks in culture, indicating that the cells did not exhibit anchorage-independent growth (100×). B. Karyotype analysis of ZYM-SIEC02 cells at passage 55, indicated that the cells contained 38 chromosomes, consistent with a diploid karyotype. C. Tumor formation assay. pSIECs and ZYM-SIEC02 cells were injected subcutaneously into nude mice. After one month, neither group of mice had developed tumors, in contrast, mice injected with EMF6 cells rapidly developed tumors within 8 days. Histological examination indicated morphologically normal tissue below the injection site of pSIECs (passage 3) and ZYM-SIEC02 cells (passage 55), but a dense cellular mass below the EMF6 cell injection site.