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ZYM-SIEC02 cells were stimulated with the indicated dose of E.coli 055:B55 LPS in the presence or absence of polymyxin B sulfate(Sigma) for 3 hours. A. Polymyxin B increased TNF-α mRNA expression when the concentration of LPS was lower than 250 ng/ml. B. Polymyxin B prevented the increase in IL-6 mRNA expression. The data shown are means±SEM of 3 independent experiments. * P<0.05;** P<0.01; *** P<0.001.
