Table 3. Effect of side chain properties of the terminal amino acid on utilization of dipeptides by the SBP penta mutant complemented with plasmids harboring an individual dipeptide-binding protein.
Dipeptide utilization by SBPsa | 2nd amino acid | Total (N-terminal) | |||||||||||||||||||
nonpolar | acidic | uncharged | |||||||||||||||||||
A1 | A2 | A3 | A4 | A5 | A1 | A2 | A3 | A4 | A5 | A1 | A2 | A3 | A4 | A5 | A1 | A2 | A3 | A4 | A5 | ||
1st amino acid | nonpolar | 32 | 34 | 27 | 32 | 1 | 11 | 11 | 11 | 10 | 0 | 10 | 10 | 9 | 9 | 0 | 53 | 55 | 47 | 51 | 1 |
acidic | 4 | 6 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 5 | 8 | 5 | 4 | 0 | |
uncharged | 7 | 7 | 7 | 7 | 1 | 5 | 5 | 5 | 5 | 0 | 2 | 2 | 2 | 2 | 0 | 14 | 14 | 14 | 14 | 1 | |
Total (C-terminal) | 43 | 47 | 38 | 42 | 2 | 16 | 16 | 16 | 15 | 0 | 13 | 14 | 12 | 12 | 0 | 72 | 77 | 66 | 69 | 2 |
The number includes only dipeptides that were not used by the SBP penta mutant.